Activity Assay:Article Title: Dusp15 modulates mtHsp70 Thr116 phosphorylation state to preserve mito-UPR and attenuate cardiac dysfunction in diabetic cardiomyopathy
Article Snippet: To assess the half-life of mtHsp70, cells were treated with cycloheximide (CHX, 100 μg/mL; Sigma-Aldrich) for various time intervals, followed by Western blot analysis. .. To inhibit Dusp15 phosphatase activity, cells were pretreated with sodium orthovanadate (SO, 10 mM, Cat. No. HY-D0852, MedChemExpress) for 6 h before HG exposure. .. Proteasome activity was modulated using either the inhibitor MG132 (2 μM, Cat. No. HY-13259, MedChemExpress) or the activator betulinic acid (10 μM, Cat. No. HY-N1451, MedChemExpress) for 6 h prior to HG treatment.
Article Title: Dusp15 modulates mtHsp70 Thr116 phosphorylation state to preserve mito-UPR and attenuate cardiac dysfunction in diabetic cardiomyopathy.
Article Snippet: To assess the half-life of mtHsp70, cells were treated with cycloheximide (CHX, 100 μg/mL; Sigma-Aldrich) for various time intervals, followed by Western blot analysis. .. To inhibit Dusp15 phosphatase activity, cells were pretreated with sodium orthovanadate (SO, 10 mM, Cat. No. HY-D0852, MedChemExpress) for 6 hours before HG exposure. .. To assess the half-life of mtHsp70, cells were treated with cycloheximide (CHX, 100 μg/mL; Sigma-Aldrich) for various time intervals, followed by Western blot analysis.
Luciferase:Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); Dual- Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENE®HD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-IB (9246S, Cell Signaling Technology); IB (9242S, Cell Signaling Technology); GST (66001- 2-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa FluorTM 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation
Article Snippet: .. The Protein A+G Agarose (P2055, Beyotime), PMSF (P7626, Sigma), Dual-Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors aprotin, leupeptin, β-glycerophosphate disodium salt, and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, and HY-D0852, MCE); 4′,6-diamidino-2-phenylindole (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); glutathione resin ( L00206 , GenScript); bovine serum albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tm (12819S, Cell Signaling Technology), and FuGENEHD (HD-1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); rabbit monoclonal antibodies against HA (H6908, Sigma); mouse monoclonal antibodies against FLAG (F3165, Sigma); horseradish peroxidase (HRP)-FLAG (ZB15939, Servicebio); rabbit polyclonal antibodies against Myc (16286-1-AP, Proteintech); HRP-His (HRP-66005, Proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, Proteintech); phosphorylated PERK (29546-1-AP, Proteintech); p-IRE1α ( Ab124945 , Abcam); IRE1α (3294S, Cell Signaling Technology); PERK (20582-1-AP, Proteintech); phosphorylated eIF2α (3398S, Cell Signaling Technology); eIF2α (11170-1-AP, Proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, Proteintech); CHOP (15204-1-AP, Proteintech); p-IκBα (9246S, Cell Signaling Technology); IκBα (9242S, Cell Signaling Technology); GST (66001-2-Ig, Proteintech); β-actin (A2228, Sigma–Aldrich); anti-mouse IgG (H+L), F(ab')2 fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology); Alexa Fluor 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); DualSpecific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENEHD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-I B (9246S, Cell Signaling Technology); I B (9242S, Cell Signaling Technology); GST (660012-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
SYBR Green Assay:Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); Dual- Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENE®HD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-IB (9246S, Cell Signaling Technology); IB (9242S, Cell Signaling Technology); GST (66001- 2-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa FluorTM 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation
Article Snippet: .. The Protein A+G Agarose (P2055, Beyotime), PMSF (P7626, Sigma), Dual-Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors aprotin, leupeptin, β-glycerophosphate disodium salt, and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, and HY-D0852, MCE); 4′,6-diamidino-2-phenylindole (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); glutathione resin ( L00206 , GenScript); bovine serum albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tm (12819S, Cell Signaling Technology), and FuGENEHD (HD-1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); rabbit monoclonal antibodies against HA (H6908, Sigma); mouse monoclonal antibodies against FLAG (F3165, Sigma); horseradish peroxidase (HRP)-FLAG (ZB15939, Servicebio); rabbit polyclonal antibodies against Myc (16286-1-AP, Proteintech); HRP-His (HRP-66005, Proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, Proteintech); phosphorylated PERK (29546-1-AP, Proteintech); p-IRE1α ( Ab124945 , Abcam); IRE1α (3294S, Cell Signaling Technology); PERK (20582-1-AP, Proteintech); phosphorylated eIF2α (3398S, Cell Signaling Technology); eIF2α (11170-1-AP, Proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, Proteintech); CHOP (15204-1-AP, Proteintech); p-IκBα (9246S, Cell Signaling Technology); IκBα (9242S, Cell Signaling Technology); GST (66001-2-Ig, Proteintech); β-actin (A2228, Sigma–Aldrich); anti-mouse IgG (H+L), F(ab')2 fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology); Alexa Fluor 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Lumpy skin disease virus protein LSDV122 impairs IFN-I receptor complex formation to evade host innate immunity
Article Snippet: .. Trypsin (C100C1, NCM Biotech), fetal bovine serum (SA211.02, Cellmax), penicillin and streptomycin ( PB180120 , Pricella), Dulbecco’s Modified Eagle Medium (C11965500BT, Gibco), puromycin (AMR-J593, VWR), protein A + G agarose (P2055, Beyotime), PMSF (P7626, Sigma), recombinant human IFN-β (300–02BC, ThermoFisher Scientific), recombinant human IFN-γ (300–02, ThermoFisher Scientific), SYBR Green Supermix (Q312, Vazyme), HiScript II Select RT SuperMix for qPCR (R323, Vazyme), and aprotin, leupeptin, β-glycerophosphate disodium salt, Ara-C, CHX and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, HY-13605, HY-12320 and HY-D0852 respectively, MCE) were purchased from the specified manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech), Rabbit monoclonal antibodies against HA (H6908, Sigma), Mouse monoclonal antibodies against Flag (AP0530, Sigma), HRP-Flag (ZB15939, Servicebio), IgG (I5381 and I5006, Sigma), p-JAK1 (3331S, Cell Signaling Technology), JAK1 (A25841, ABclonal), p-TYK2 and TYK2 (9321S and 14193S, Cell Signaling Technology), p-STAT1 (9167S, Cell Signaling Technology), STAT1 (sc-417, Santa Cruz Biotechnology), p-STAT2 and STAT2 (88410S and 72604S, Cell Signaling Technology), IFNAR1 (A0575, ABclonal), IFNAR2 (53883s, Cell Signaling Technology), PE anti-DYKDDDDK Tag (Biolegend, 637310), Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology) and Alexa Fluor 488 goat anti-rabbit IgG (H + L) (A11008, Invitrogen) were purchased from the specified manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); DualSpecific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENEHD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-I B (9246S, Cell Signaling Technology); I B (9242S, Cell Signaling Technology); GST (660012-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Real-time Polymerase Chain Reaction:Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); Dual- Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENE®HD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-IB (9246S, Cell Signaling Technology); IB (9242S, Cell Signaling Technology); GST (66001- 2-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa FluorTM 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation
Article Snippet: .. The Protein A+G Agarose (P2055, Beyotime), PMSF (P7626, Sigma), Dual-Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors aprotin, leupeptin, β-glycerophosphate disodium salt, and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, and HY-D0852, MCE); 4′,6-diamidino-2-phenylindole (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); glutathione resin ( L00206 , GenScript); bovine serum albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tm (12819S, Cell Signaling Technology), and FuGENEHD (HD-1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); rabbit monoclonal antibodies against HA (H6908, Sigma); mouse monoclonal antibodies against FLAG (F3165, Sigma); horseradish peroxidase (HRP)-FLAG (ZB15939, Servicebio); rabbit polyclonal antibodies against Myc (16286-1-AP, Proteintech); HRP-His (HRP-66005, Proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, Proteintech); phosphorylated PERK (29546-1-AP, Proteintech); p-IRE1α ( Ab124945 , Abcam); IRE1α (3294S, Cell Signaling Technology); PERK (20582-1-AP, Proteintech); phosphorylated eIF2α (3398S, Cell Signaling Technology); eIF2α (11170-1-AP, Proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, Proteintech); CHOP (15204-1-AP, Proteintech); p-IκBα (9246S, Cell Signaling Technology); IκBα (9242S, Cell Signaling Technology); GST (66001-2-Ig, Proteintech); β-actin (A2228, Sigma–Aldrich); anti-mouse IgG (H+L), F(ab')2 fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology); Alexa Fluor 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Lumpy skin disease virus protein LSDV122 impairs IFN-I receptor complex formation to evade host innate immunity
Article Snippet: .. Trypsin (C100C1, NCM Biotech), fetal bovine serum (SA211.02, Cellmax), penicillin and streptomycin ( PB180120 , Pricella), Dulbecco’s Modified Eagle Medium (C11965500BT, Gibco), puromycin (AMR-J593, VWR), protein A + G agarose (P2055, Beyotime), PMSF (P7626, Sigma), recombinant human IFN-β (300–02BC, ThermoFisher Scientific), recombinant human IFN-γ (300–02, ThermoFisher Scientific), SYBR Green Supermix (Q312, Vazyme), HiScript II Select RT SuperMix for qPCR (R323, Vazyme), and aprotin, leupeptin, β-glycerophosphate disodium salt, Ara-C, CHX and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, HY-13605, HY-12320 and HY-D0852 respectively, MCE) were purchased from the specified manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech), Rabbit monoclonal antibodies against HA (H6908, Sigma), Mouse monoclonal antibodies against Flag (AP0530, Sigma), HRP-Flag (ZB15939, Servicebio), IgG (I5381 and I5006, Sigma), p-JAK1 (3331S, Cell Signaling Technology), JAK1 (A25841, ABclonal), p-TYK2 and TYK2 (9321S and 14193S, Cell Signaling Technology), p-STAT1 (9167S, Cell Signaling Technology), STAT1 (sc-417, Santa Cruz Biotechnology), p-STAT2 and STAT2 (88410S and 72604S, Cell Signaling Technology), IFNAR1 (A0575, ABclonal), IFNAR2 (53883s, Cell Signaling Technology), PE anti-DYKDDDDK Tag (Biolegend, 637310), Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology) and Alexa Fluor 488 goat anti-rabbit IgG (H + L) (A11008, Invitrogen) were purchased from the specified manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); DualSpecific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENEHD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-I B (9246S, Cell Signaling Technology); I B (9242S, Cell Signaling Technology); GST (660012-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Cloning:Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); Dual- Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENE®HD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-IB (9246S, Cell Signaling Technology); IB (9242S, Cell Signaling Technology); GST (66001- 2-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa FluorTM 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation
Article Snippet: .. The Protein A+G Agarose (P2055, Beyotime), PMSF (P7626, Sigma), Dual-Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors aprotin, leupeptin, β-glycerophosphate disodium salt, and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, and HY-D0852, MCE); 4′,6-diamidino-2-phenylindole (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); glutathione resin ( L00206 , GenScript); bovine serum albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tm (12819S, Cell Signaling Technology), and FuGENEHD (HD-1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); rabbit monoclonal antibodies against HA (H6908, Sigma); mouse monoclonal antibodies against FLAG (F3165, Sigma); horseradish peroxidase (HRP)-FLAG (ZB15939, Servicebio); rabbit polyclonal antibodies against Myc (16286-1-AP, Proteintech); HRP-His (HRP-66005, Proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, Proteintech); phosphorylated PERK (29546-1-AP, Proteintech); p-IRE1α ( Ab124945 , Abcam); IRE1α (3294S, Cell Signaling Technology); PERK (20582-1-AP, Proteintech); phosphorylated eIF2α (3398S, Cell Signaling Technology); eIF2α (11170-1-AP, Proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, Proteintech); CHOP (15204-1-AP, Proteintech); p-IκBα (9246S, Cell Signaling Technology); IκBα (9242S, Cell Signaling Technology); GST (66001-2-Ig, Proteintech); β-actin (A2228, Sigma–Aldrich); anti-mouse IgG (H+L), F(ab')2 fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology); Alexa Fluor 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); DualSpecific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENEHD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-I B (9246S, Cell Signaling Technology); I B (9242S, Cell Signaling Technology); GST (660012-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Polymerase Chain Reaction:Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); Dual- Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENE®HD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-IB (9246S, Cell Signaling Technology); IB (9242S, Cell Signaling Technology); GST (66001- 2-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa FluorTM 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation
Article Snippet: .. The Protein A+G Agarose (P2055, Beyotime), PMSF (P7626, Sigma), Dual-Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors aprotin, leupeptin, β-glycerophosphate disodium salt, and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, and HY-D0852, MCE); 4′,6-diamidino-2-phenylindole (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); glutathione resin ( L00206 , GenScript); bovine serum albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tm (12819S, Cell Signaling Technology), and FuGENEHD (HD-1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); rabbit monoclonal antibodies against HA (H6908, Sigma); mouse monoclonal antibodies against FLAG (F3165, Sigma); horseradish peroxidase (HRP)-FLAG (ZB15939, Servicebio); rabbit polyclonal antibodies against Myc (16286-1-AP, Proteintech); HRP-His (HRP-66005, Proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, Proteintech); phosphorylated PERK (29546-1-AP, Proteintech); p-IRE1α ( Ab124945 , Abcam); IRE1α (3294S, Cell Signaling Technology); PERK (20582-1-AP, Proteintech); phosphorylated eIF2α (3398S, Cell Signaling Technology); eIF2α (11170-1-AP, Proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, Proteintech); CHOP (15204-1-AP, Proteintech); p-IκBα (9246S, Cell Signaling Technology); IκBα (9242S, Cell Signaling Technology); GST (66001-2-Ig, Proteintech); β-actin (A2228, Sigma–Aldrich); anti-mouse IgG (H+L), F(ab')2 fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology); Alexa Fluor 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); DualSpecific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENEHD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-I B (9246S, Cell Signaling Technology); I B (9242S, Cell Signaling Technology); GST (660012-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Protein Purification:Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); Dual- Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENE®HD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-IB (9246S, Cell Signaling Technology); IB (9242S, Cell Signaling Technology); GST (66001- 2-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa FluorTM 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation
Article Snippet: .. The Protein A+G Agarose (P2055, Beyotime), PMSF (P7626, Sigma), Dual-Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors aprotin, leupeptin, β-glycerophosphate disodium salt, and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, and HY-D0852, MCE); 4′,6-diamidino-2-phenylindole (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); glutathione resin ( L00206 , GenScript); bovine serum albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tm (12819S, Cell Signaling Technology), and FuGENEHD (HD-1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); rabbit monoclonal antibodies against HA (H6908, Sigma); mouse monoclonal antibodies against FLAG (F3165, Sigma); horseradish peroxidase (HRP)-FLAG (ZB15939, Servicebio); rabbit polyclonal antibodies against Myc (16286-1-AP, Proteintech); HRP-His (HRP-66005, Proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, Proteintech); phosphorylated PERK (29546-1-AP, Proteintech); p-IRE1α ( Ab124945 , Abcam); IRE1α (3294S, Cell Signaling Technology); PERK (20582-1-AP, Proteintech); phosphorylated eIF2α (3398S, Cell Signaling Technology); eIF2α (11170-1-AP, Proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, Proteintech); CHOP (15204-1-AP, Proteintech); p-IκBα (9246S, Cell Signaling Technology); IκBα (9242S, Cell Signaling Technology); GST (66001-2-Ig, Proteintech); β-actin (A2228, Sigma–Aldrich); anti-mouse IgG (H+L), F(ab')2 fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology); Alexa Fluor 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Article Title: Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammation.
Article Snippet: .. The Protein A+G agarose (P2055, Beyotime); PMSF (P7626, Sigma); DualSpecific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme); the ClonExpress Ultra One Step Cloning Kit for PCR (C115, Vazyme); the inhibitors Aprotin, leupeptin, -glycerophosphate disodium salt and sodium orthovanadate (HYP0017, HY-18234A, HY-126304, and HY-D0852, MCE); DAPI (C1002, Beyotime); His-tag protein purification kit (P2226, Beyotime); Glutathione Resin (L00206, GenScript); Bovine Serum Albumin (A8020, Solarbio); RNAiso Plus reagent (9109, Takara); Tunicamycin (12819s, Cell Signaling Technology), and FuGENEHD (HD1000, Promega) were purchased from the indicated manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech); Rabbit monoclonal antibodies against HA (H6908, Sigma); Mouse monoclonal antibodies Jo urn al Pr e-p roo f against Flag (F3165, Sigma); HRP-Flag (ZB15939, Servicebio); Rabbit Polyclonal antibodies against Myc (16286-1-AP, proteintech); HRP-His (HRP-66005, proteintech); IgG (I5381 and I5006, Sigma); BiP (11587-1-AP, proteintech); p-PERK (29546-1-AP, proteintech); p-IRE1 (Ab124945, Abcam); IRE1 (3294S, Cell Signaling Technology); PERK (20582-1-AP, proteintech); p-eIF2 (3398S, Cell Signaling Technology); eIF2 (11170-1-AP, proteintech); ATF6 (ab122897, Abcam); XBP1s (24868-1-AP, proteintech); CHOP (15204-1-AP, proteintech); p-I B (9246S, Cell Signaling Technology); I B (9242S, Cell Signaling Technology); GST (660012-Ig, proteintech ); -actin (A2228, Sigma-Aldrich); Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (8890, Cell Signaling Technology); Alexa FluorTM 555 goat anti-rabbit IgG (H+L) (A-21428, Invitrogen), and Alexa Fluor 488 goat anti-rabbit IgG (H+L) (A11008, Invitrogen) were purchased from the indicated manufacturers.
Modification:Article Title: Lumpy skin disease virus protein LSDV122 impairs IFN-I receptor complex formation to evade host innate immunity
Article Snippet: .. Trypsin (C100C1, NCM Biotech), fetal bovine serum (SA211.02, Cellmax), penicillin and streptomycin ( PB180120 , Pricella), Dulbecco’s Modified Eagle Medium (C11965500BT, Gibco), puromycin (AMR-J593, VWR), protein A + G agarose (P2055, Beyotime), PMSF (P7626, Sigma), recombinant human IFN-β (300–02BC, ThermoFisher Scientific), recombinant human IFN-γ (300–02, ThermoFisher Scientific), SYBR Green Supermix (Q312, Vazyme), HiScript II Select RT SuperMix for qPCR (R323, Vazyme), and aprotin, leupeptin, β-glycerophosphate disodium salt, Ara-C, CHX and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, HY-13605, HY-12320 and HY-D0852 respectively, MCE) were purchased from the specified manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech), Rabbit monoclonal antibodies against HA (H6908, Sigma), Mouse monoclonal antibodies against Flag (AP0530, Sigma), HRP-Flag (ZB15939, Servicebio), IgG (I5381 and I5006, Sigma), p-JAK1 (3331S, Cell Signaling Technology), JAK1 (A25841, ABclonal), p-TYK2 and TYK2 (9321S and 14193S, Cell Signaling Technology), p-STAT1 (9167S, Cell Signaling Technology), STAT1 (sc-417, Santa Cruz Biotechnology), p-STAT2 and STAT2 (88410S and 72604S, Cell Signaling Technology), IFNAR1 (A0575, ABclonal), IFNAR2 (53883s, Cell Signaling Technology), PE anti-DYKDDDDK Tag (Biolegend, 637310), Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology) and Alexa Fluor 488 goat anti-rabbit IgG (H + L) (A11008, Invitrogen) were purchased from the specified manufacturers.
Recombinant:Article Title: Lumpy skin disease virus protein LSDV122 impairs IFN-I receptor complex formation to evade host innate immunity
Article Snippet: .. Trypsin (C100C1, NCM Biotech), fetal bovine serum (SA211.02, Cellmax), penicillin and streptomycin ( PB180120 , Pricella), Dulbecco’s Modified Eagle Medium (C11965500BT, Gibco), puromycin (AMR-J593, VWR), protein A + G agarose (P2055, Beyotime), PMSF (P7626, Sigma), recombinant human IFN-β (300–02BC, ThermoFisher Scientific), recombinant human IFN-γ (300–02, ThermoFisher Scientific), SYBR Green Supermix (Q312, Vazyme), HiScript II Select RT SuperMix for qPCR (R323, Vazyme), and aprotin, leupeptin, β-glycerophosphate disodium salt, Ara-C, CHX and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, HY-13605, HY-12320 and HY-D0852 respectively, MCE) were purchased from the specified manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech), Rabbit monoclonal antibodies against HA (H6908, Sigma), Mouse monoclonal antibodies against Flag (AP0530, Sigma), HRP-Flag (ZB15939, Servicebio), IgG (I5381 and I5006, Sigma), p-JAK1 (3331S, Cell Signaling Technology), JAK1 (A25841, ABclonal), p-TYK2 and TYK2 (9321S and 14193S, Cell Signaling Technology), p-STAT1 (9167S, Cell Signaling Technology), STAT1 (sc-417, Santa Cruz Biotechnology), p-STAT2 and STAT2 (88410S and 72604S, Cell Signaling Technology), IFNAR1 (A0575, ABclonal), IFNAR2 (53883s, Cell Signaling Technology), PE anti-DYKDDDDK Tag (Biolegend, 637310), Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology) and Alexa Fluor 488 goat anti-rabbit IgG (H + L) (A11008, Invitrogen) were purchased from the specified manufacturers.
|
MedChemExpress is a verified supplier 
MedChemExpress manufactures this product 